Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias

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OBJECTIVES: The aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21WAF1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias.MATERIAL AND METHODS: This Other Pet Care Products was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias.excisional biopsies were performed and the patients remain under clinical follow-up.The leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias.

Paraffin-embebeded sections were immunostained for p53 and p21WAF1.Five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage Supplements of positive cells.A qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers.Groups were compared with ANOVA (p0.

05).CONCLUSIONS: Our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21WAF1 immunostaining, notwithstanding similar profiles between non-dysplastic and dysplastic leukoplakias were observed.